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pLFYalpha
(Plasmid #243804)

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  • Purpose
    (Empty Backbone) Dropout vector for genomic integration into yeast strain LFYalpha. GFP is flanked by BamHI sites for easy cloning using Gibson assembly. Construct contains attB site for the serine recombinase BxB1.
  • Depositing Lab
    Sai Reddy
  • Publication
    Lester et al bioRxiv ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 243804 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pYTK090
  • Backbone manufacturer
    John Dueber (Addgene #65197)
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains sfGFP and partial Trp1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLFYalpha was a gift from Sai Reddy (Addgene plasmid # 243804 ; http://n2t.net/addgene:243804 ; RRID:Addgene_243804)

  • For your References section:

    A yeast mating-based platform enables the generation and screening of ultra large antibody libraries. Lester Frei, Sai Tosa Reddy. bioRxiv 10.1101/2025.10.06.680701
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