Skip to main content
Addgene

GST-Thrombin-Munc13-1 MUN domain (aa859-1516 delta1408-1452 EF)
(Plasmid #243818)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 243818 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pGEX
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Munc13-1
  • Species
    R. norvegicus (rat)
  • Mutation
    1408-1452 --> EF
  • Entrez Gene
    Unc13a (a.k.a. Munc13-1, Unc13h1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NCBI Reference Sequence: NM_022861.2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-Thrombin-Munc13-1 MUN domain (aa859-1516 delta1408-1452 EF) was a gift from Jose Rizo-Rey (Addgene plasmid # 243818 ; http://n2t.net/addgene:243818 ; RRID:Addgene_243818)

  • For your References section:

    Membrane bridging by Munc13-1 is crucial for neurotransmitter release. Quade B, Camacho M, Zhao X, Orlando M, Trimbuch T, Xu J, Li W, Nicastro D, Rosenmund C, Rizo J. Elife. 2019 Feb 28;8:e42806. doi: 10.7554/eLife.42806. 10.7554/eLife.42806 PubMed 30816091
Related items: