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His-Thrombin-Munc13-1 C1-C2B-MUN-C2C R1598E F1658E (delta1408-1452 delta1533-1551)
(Plasmid #243822)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 243822 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pet28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Munc13-1
  • Species
    R. norvegicus (rat)
  • Mutation
    delta1408-1452 delta1533-1551 R1598E F1658E
  • Entrez Gene
    Unc13a (a.k.a. Munc13-1, Unc13h1)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Other

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NCBI Reference Sequence: NM_022861.2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-Thrombin-Munc13-1 C1-C2B-MUN-C2C R1598E F1658E (delta1408-1452 delta1533-1551) was a gift from Jose Rizo-Rey (Addgene plasmid # 243822 ; http://n2t.net/addgene:243822 ; RRID:Addgene_243822)

  • For your References section:

    Membrane bridging by Munc13-1 is crucial for neurotransmitter release. Quade B, Camacho M, Zhao X, Orlando M, Trimbuch T, Xu J, Li W, Nicastro D, Rosenmund C, Rizo J. Elife. 2019 Feb 28;8:e42806. doi: 10.7554/eLife.42806. 10.7554/eLife.42806 PubMed 30816091
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