pYPQ230-AtEXO1B-N
(Plasmid
#244024)
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PurposeGateway entry plasmid (attL1& attR5) expressing AtEXO1B exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 244024 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepYPQ230
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Backbone manufacturerYiping Qi
- Total vector size (bp) 9254
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas12a
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Alt nameLbCas12a
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SpeciesA. thaliana (mustard weed); L. bacterium
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Insert Size (bp)2203
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Tag
/ Fusion Protein
- AtEXO1B (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ230-AtEXO1B-N was a gift from Yiping Qi (Addgene plasmid # 244024 ; http://n2t.net/addgene:244024 ; RRID:Addgene_244024) -
For your References section:
Increasing Cas12a- and Cas9-mediated deletion sizes with exonucleases. Wang X, Clem J, Malzahn A, Qi Y. Plant Physiol. 2025 Oct 31;199(3):kiaf507. doi: 10.1093/plphys/kiaf507. 10.1093/plphys/kiaf507 PubMed 41182408