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pMITO-mTFP1
(Plasmid #244030)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244030 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA4TO
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CISD1-mTFP1
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • sunTagV4 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMITO-mTFP1 was a gift from Markus Affolter (Addgene plasmid # 244030 ; http://n2t.net/addgene:244030 ; RRID:Addgene_244030)

  • For your References section:

    DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo protein manipulations. Vigano MA, Bieli D, Schaefer JV, Jakob RP, Matsuda S, Maier T, Pluckthun A, Affolter M. Biol Open. 2018 Oct 29;7(11). pii: bio.036749. doi: 10.1242/bio.036749. 10.1242/bio.036749 PubMed 30237292
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