pUASTattBVhhGFP4-Cry2
(Plasmid
#244065)
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PurposeantiGFP-nanobody fused to the light sensitive dimerization domain CRY2
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 244065 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUASattB
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameVhhGFP4-Cry2-myc
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SpeciesSynthetic
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Tag
/ Fusion Protein
- myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUASTattBVhhGFP4-Cry2 was a gift from Markus Affolter (Addgene plasmid # 244065 ; http://n2t.net/addgene:244065 ; RRID:Addgene_244065) -
For your References section:
In vivo regulation of an endogenously tagged protein by a light-regulated kinase. Bao M, Lepeta K, Aguilar G, Schnider S, Affolter M, Vigano MA. G3 (Bethesda). 2025 Jun 4;15(6):jkaf073. doi: 10.1093/g3journal/jkaf073. 10.1093/g3journal/jkaf073 PubMed 40194518