Skip to main content
Addgene
Open Menu

pUC-GW-Kan-VPC
(Plasmid #244163)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244163 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pUC57--GW-Kan
  • Backbone manufacturer
    Genewiz
  • Backbone size w/o insert (bp) 2871
  • Total vector size (bp) 6024
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Just Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAPK14/p38a
  • Alt name
    MAPK
  • Alt name
    p38a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1080
  • GenBank ID
    L35263.1 NM_001315
  • Entrez Gene
    MAPK14 (a.k.a. CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA)
  • Promoter CMV promoter
  • Tags / Fusion Proteins
    • mVenus (N terminal on insert)
    • mCerulean3 (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer taatacgactcactatagagg
  • 3′ sequencing primer aactggttcttctctacctcagg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC-GW-Kan-VPC was a gift from Thomas Kuhlman (Addgene plasmid # 244163 ; http://n2t.net/addgene:244163 ; RRID:Addgene_244163)
Related items: