L4804 VEGFR1 CTEVp chain in PolyTX-mTagBFP2
(Plasmid
#244174)
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PurposeConstitutive expression of a MESA CTEVp chain encoding (N to C): VEGFR1SS-3xFLAG-VEGFR1ECD-VEGFR1TMD-CTEVp(190K)-PRS(M)-VP64-ZF6
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 244174 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepPD1053 Polytransfect L1 mTagBFP2
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMESA CTEVp chain with human VEGFR1 SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6)
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SpeciesH. sapiens (human), Synthetic
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MutationCTEVp_190K
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Entrez GeneFLT1 (a.k.a. FLT, FLT-1, VEGFR-1, VEGFR1)
- Promoter hEF1a
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Tag
/ Fusion Protein
- VEGFR1 signal peptide - 3xFLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.03.23.586421 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
L4804 VEGFR1 CTEVp chain in PolyTX-mTagBFP2 was a gift from Joshua Leonard (Addgene plasmid # 244174 ; http://n2t.net/addgene:244174 ; RRID:Addgene_244174) -
For your References section:
Conversion of natural cytokine receptors into orthogonal synthetic biosensors. Edelstein HI, Cosio A, Ezekiel ML, Corcoran WK, Morris AH, Leonard JN. Nat Chem Biol. 2025 Aug 22:10.1038/s41589-025-01986-1. doi: 10.1038/s41589-025-01986-1. 10.1038/s41589-025-01986-1 PubMed 40846994