Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2
(Plasmid #24419)

• Purpose: Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Expresses mCherry and Rs1 via P2A ribosomal skip sequence. Expresses tTA under EF1α promoter. Rosa26 homology arms for integration.
• Depositing Lab: Edward Hsiao
• Publication: Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Backbone

• Vector backbone: pRosa26
• Backbone manufacturer: Plasmid 21714: pROSA26-1 (Soriano Lab)
• Backbone size w/o insert (bp): 4486
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: RS1
• Species: H. sapiens (human)
• Insert Size (bp): 1166
• Mutation: D100A
• Entrez Gene: HTR4 (a.k.a. 5-HT4, 5-HT4R)
• Promoter: TRE, miniCMV
• Tags / Fusion Proteins:
  • mCherry-P2A (N terminal on insert)
  • FLAG (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: DsRed1-C

Gene/Insert 2

• Gene/Insert name: tTA
• Promoter: EF1α

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Rosa26 targeting vector containing the EF1α-tTA/TetO-mCh-Rs1 cassette flanked by insulator sequences.
Plasmid also known as Exp-R26(EF1α-tTA/TetO-mCh-Rs1).