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L4866 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF1a in MGEV
(Plasmid #244190)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244190 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pPD1179 Multigene Expression Destination Vector
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mTagBFP2; IL-10Rb NTEVp chain with human IgG VH signal sequence; IL-10Ra CTEVp chain with ZF1 synTF
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    NTEVp_75S, CTEVp_190K
  • Promoter hEF1a, CMV
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.23.586421 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L4866 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF1a in MGEV was a gift from Joshua Leonard (Addgene plasmid # 244190 ; http://n2t.net/addgene:244190 ; RRID:Addgene_244190)

  • For your References section:

    Conversion of natural cytokine receptors into orthogonal synthetic biosensors. Edelstein HI, Cosio A, Ezekiel ML, Corcoran WK, Morris AH, Leonard JN. Nat Chem Biol. 2025 Aug 22:10.1038/s41589-025-01986-1. doi: 10.1038/s41589-025-01986-1. 10.1038/s41589-025-01986-1 PubMed 40846994
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