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L4505 x6TRE2 (cJun2) Ben DsRed-Express2 in TUPV1
(Plasmid #244200)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244200 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    TUPV1
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cJun-responsive promoter (6 cJun homodimer binding sites + YB_TATA), dsRed-Express2 reporter
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.23.586421 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L4505 x6TRE2 (cJun2) Ben DsRed-Express2 in TUPV1 was a gift from Joshua Leonard (Addgene plasmid # 244200 ; http://n2t.net/addgene:244200 ; RRID:Addgene_244200)

  • For your References section:

    Conversion of natural cytokine receptors into orthogonal synthetic biosensors. Edelstein HI, Cosio A, Ezekiel ML, Corcoran WK, Morris AH, Leonard JN. Nat Chem Biol. 2025 Aug 22:10.1038/s41589-025-01986-1. doi: 10.1038/s41589-025-01986-1. 10.1038/s41589-025-01986-1 PubMed 40846994
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