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pAAVLINKv1-pCALM1-Cre-3'-TOP2B-FLAG
(Plasmid #244335)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244335 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV-MCS2
  • Backbone manufacturer
    Steve Jackson (Addgene # 46954)
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TOP2B
  • Species
    H. sapiens (human)
  • Entrez Gene
    TOP2B (a.k.a. BILU, TOPIIB, top2beta)
  • Promoter pCALM1
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See publication for corresponding AAV Link vectors.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAVLINKv1-pCALM1-Cre-3'-TOP2B-FLAG was a gift from Zhonghua Lu (Addgene plasmid # 244335 ; http://n2t.net/addgene:244335 ; RRID:Addgene_244335)

  • For your References section:

    AAVLINK: A potent DNA-recombination method for large cargo delivery in gene therapy. Lin J, Lin Y, Liu N, Cao W, Zhang J, Wen S, Zhang Y, Liao W, Hong Z, Lin Y, Liu Q, Liu H, Li Q, Chen B, Li M, Luo Z, Yang L, Yang Y, Zheng SH, Wang Y, Chu H, Hu Y, Qin Y, Luo BX, Tian S, Chen Y, Yan T, Yang L, Wang H, Liu T, Jiang Y, Lu Z. Cell. 2026 Feb 5;189(3):969-986.e17. doi: 10.1016/j.cell.2025.12.039. Epub 2026 Jan 27. 10.1016/j.cell.2025.12.039 PubMed 41605211
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