pKH9-catP
(Plasmid
#244450)
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PurposeA shuttle plasmid compatible with Fusobacterium nucleatum ATCC10953 or ATCC25586, carrying a thiamphenicol resistance marker (catP gene).
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 244450 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUC19
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Backbone manufacturerhomemade
- Backbone size w/o insert (bp) 2379
- Total vector size (bp) 3953
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namepKH09 Fn ori
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SpeciesSynthetic
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Insert Size (bp)956
Gene/Insert 2
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Gene/Insert nameCatP
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Alt namecat-opt-Fn
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SpeciesSynthetic
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Insert Size (bp)624
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.07.17.603877 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKH9-catP was a gift from Xue Liu (Addgene plasmid # 244450 ; http://n2t.net/addgene:244450 ; RRID:Addgene_244450)