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pLKO.1C-YFP-shRB1-733
(Plasmid #244458)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244458 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLKO.1C
  • Vector type
    Lentiviral
  • Selectable markers
    YFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shRB1
  • gRNA/shRNA sequence
    tttggactagaaataatgtgg
  • Species
    H. sapiens (human)
  • Entrez Gene
    RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1C-YFP-shRB1-733 was a gift from David Cobrinik (Addgene plasmid # 244458 ; http://n2t.net/addgene:244458 ; RRID:Addgene_244458)

  • For your References section:

    Improved third-generation lentiviral packaging with pLKO.1C vectors. Lee S, Cobrinik D. Biotechniques. 2020 Jun;68(6):349-352. doi: 10.2144/btn-2019-0155. Epub 2020 Mar 6. 10.2144/btn-2019-0155 PubMed 32141762
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