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Addgene

DoS3 plasmid
(Plasmid #244471)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244471 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    R+ from A. J. Lopatkin, et al., Antibiotics as a selective driver for conjugation dynamics. Nat. Microbiol. 1, 16044 (2016)
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    I-SceI enzyme
  • Species
    Synthetic
  • Insert Size (bp)
    708
  • Entrez Gene
    CSS3 (a.k.a. YOL159C)
  • Promoter araBAD promoter

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DoS3 plasmid was a gift from Lingchong You (Addgene plasmid # 244471 ; http://n2t.net/addgene:244471 ; RRID:Addgene_244471)

  • For your References section:

    A predatory gene drive for targeted control of self-transmissible plasmids. Tsoi R, Son HI, Hamrick GS, Tang K, Bethke JH, Lu J, Maddamsetti R, You L. Sci Adv. 2025 Apr 4;11(14):eads4735. doi: 10.1126/sciadv.ads4735. Epub 2025 Apr 2. 10.1126/sciadv.ads4735 PubMed 40173243
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