CL20_mEGFP_FOidr_157
(Plasmid
#244709)
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PurposeExpress mEGFP-tagged intrinsically disordered protein (IDR), FOidr_157, derived from human fusion protein PPP2CA_CDKL3
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 244709 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneCL20
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFOidr_157
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SpeciesH. sapiens (human)
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MutationUnmutated
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Tag
/ Fusion Protein
- monomeric EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PPP2CA_CDKL3_29_130
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CL20_mEGFP_FOidr_157 was a gift from Richard Kriwacki (Addgene plasmid # 244709 ; http://n2t.net/addgene:244709 ; RRID:Addgene_244709)