pBSII-NLS-mTagBFP(AM)
(Plasmid #244808)

• Purpose: In-Vitro Synthesis of mRNA encoding a Honeybee-optimized mTagBFP2-labeled Nuclear Localization Signal (NLS) Tag
• Depositing Lab: Frederic Strobl
• Publication: In-Vitro Synthesis of mRNA for Fluorescent Labeling of Honeybee Embryos (unpublished)

Backbone

• Vector backbone: pBluescript II SK (+)
• Total vector size (bp): 4661
• Vector type: In-Vitro mRNA Synthesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mTagBFP2
• Species: Synthetic
• Mutation: coding sequence is optimized for the Honeybee
• Promoter: T7
• Tag / Fusion Protein:
  • SV40 NLS (N terminal on insert)

Cloning Information

• Cloning method: Other
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • Plasmid - pBSII-NLS-mTagBFP(AM).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry