pBSII-LA-mCherry(AM)
(Plasmid
#244815)
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PurposeIn-Vitro Synthesis of mRNA encoding a Honeybee-optimized mCherry-labeled Lifeact (LA) Tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 244815 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepBluescript II SK (+)
- Total vector size (bp) 4679
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Vector typeIn-Vitro mRNA Synthesis
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry
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SpeciesS. cerevisiae (budding yeast); Discosoma sp.
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Mutationcoding sequence is optimized for the Honeybee
- Promoter T7
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Tag
/ Fusion Protein
- Lifeact (N terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSII-LA-mCherry(AM) was a gift from Frederic Strobl (Addgene plasmid # 244815 ; http://n2t.net/addgene:244815 ; RRID:Addgene_244815)
Map uploaded by the depositor.
