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pHS25_10xHis_MBP_TEV_EQR-Cas9
(Plasmid #244828)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244828 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    p2CT
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EQR Cas9
  • Species
    Streptococcus pyogenes
  • Mutation
    D1135E/R1335Q/T1337R
  • Promoter T7
  • Tag / Fusion Protein
    • 10xHis-MBP (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHS25_10xHis_MBP_TEV_EQR-Cas9 was a gift from Jennifer Doudna (Addgene plasmid # 244828 ; http://n2t.net/addgene:244828 ; RRID:Addgene_244828)

  • For your References section:

    Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Shi H, Al-Sayyad N, Wasko KM, Trinidad MI, Doherty EE, Vohra K, Boger RS, Colognori D, Cofsky JC, Skopintsev P, Bryant Z, Doudna JA. Mol Cell. 2025 May 1;85(9):1730-1742.e9. doi: 10.1016/j.molcel.2025.03.024. Epub 2025 Apr 23. 10.1016/j.molcel.2025.03.024 PubMed 40273916
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