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Addgene

pHS638_Flex-Cas12a-CBE
(Plasmid #244845)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244845 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    PX458
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flex-dCas12a-CBE
  • Species
    Lachnospiraceae bacterium
  • Mutation
    G146R/R182V/D535G/S551F/D665N/E795Q/D832A
  • Promoter CMV
  • Tags / Fusion Proteins
    • 2xSV40 NLS-APOBEC3A (N terminal on insert)
    • Nucleoplasmid NLS-UGI-SV40 NLS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHS638_Flex-Cas12a-CBE was a gift from Jennifer Doudna (Addgene plasmid # 244845 ; http://n2t.net/addgene:244845 ; RRID:Addgene_244845)

  • For your References section:

    Directed evolution expands CRISPR-Cas12a genome-editing capacity. Ma E, Chen K, Shi H, Wasko KM, Esain-Garcia I, Trinidad MI, Zhou K, Ye J, Doudna JA. Nucleic Acids Res. 2025 Jul 8;53(13):gkaf649. doi: 10.1093/nar/gkaf649. 10.1093/nar/gkaf649 PubMed 40671523
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