pHS641_Flex-Cas12a-ABE
(Plasmid
#244846)
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PurposeMammalian expression of Flex-Cas12a adenine base editor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 244846 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonePX458
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFlex-dCas12a-ABE
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SpeciesLachnospiraceae bacterium
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MutationG146R/R182V/D535G/S551F/D665N/E795Q/D832A
- Promoter CMV
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Tag
/ Fusion Protein
- SV40 NLS-ABE8e (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHS641_Flex-Cas12a-ABE was a gift from Jennifer Doudna (Addgene plasmid # 244846 ; http://n2t.net/addgene:244846 ; RRID:Addgene_244846) -
For your References section:
Directed evolution expands CRISPR-Cas12a genome-editing capacity. Ma E, Chen K, Shi H, Wasko KM, Esain-Garcia I, Trinidad MI, Zhou K, Ye J, Doudna JA. Nucleic Acids Res. 2025 Jul 8;53(13):gkaf649. doi: 10.1093/nar/gkaf649. 10.1093/nar/gkaf649 PubMed 40671523