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pTwist Amp High Copy ENDOG-HA
(Plasmid #244881)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244881 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTwist Amp High Copy
  • Backbone manufacturer
    Twist Biosciences
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ENDOG
  • Species
    H. sapiens (human)
  • Entrez Gene
    ENDOG
  • Promoter SP6
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Twist Biosciences

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SP6 promoter cloned upstream of the gene for in vitro transcription.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTwist Amp High Copy ENDOG-HA was a gift from Alexis Jourdain (Addgene plasmid # 244881 ; http://n2t.net/addgene:244881 ; RRID:Addgene_244881)

  • For your References section:

    An updated inventory of genes essential for oxidative phosphorylation identifies a mitochondrial origin in familial Meniere's disease. Harhai M, Foged MM, Zarges C, Landoni JC, Chollet S, Simonelli M, Recazens E, Lisci M, Laban N, Manley S, Riemer J, Lopez-Escamez JA, Lysakowski A, Jourdain AA. Cell Rep. 2025 Jul 25;44(8):116069. doi: 10.1016/j.celrep.2025.116069. 10.1016/j.celrep.2025.116069 PubMed 40714634
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