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Addgene

EF1a_eeBxb1_Int_2x_NPL_c-term_NLS_pTwist
(Plasmid #244901)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244901 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pUC-GW-Amp
  • Backbone manufacturer
    Genewiz
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eeBxb1
  • Species
    Synthetic
  • Promoter EF1a

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.08.29.610246 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EF1a_eeBxb1_Int_2x_NPL_c-term_NLS_pTwist was a gift from Andrew Bassett (Addgene plasmid # 244901)
  • For your References section:

    ONE-STEP tagging: a versatile method for rapid site-specific integration by simultaneous reagent delivery. Migliori V, Bruntraeger MB, Guylev IS, Burgold T, Lichou F, Trinh AL, Washer SJ, Jones CP, Trynka G, Bassett AR. bioRxiv 2024.08.29.610246 10.1101/2024.08.29.610246