EF1a_eeBxb1_Int_2x_NPL_c-term_NLS_pTwist
(Plasmid
#244901)
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PurposeExpression of eeBxb1
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 244901 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepUC-GW-Amp
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Backbone manufacturerGenewiz
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeeBxb1
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SpeciesSynthetic
- Promoter EF1a
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EF1a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.08.29.610246 for the bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EF1a_eeBxb1_Int_2x_NPL_c-term_NLS_pTwist was a gift from Andrew Bassett (Addgene plasmid # 244901) -
For your References section:
ONE-STEP tagging: a versatile method for rapid site-specific integration by simultaneous reagent delivery. Migliori V, Bruntraeger MB, Guylev IS, Burgold T, Lichou F, Trinh AL, Washer SJ, Jones CP, Trynka G, Bassett AR. bioRxiv 2024.08.29.610246 10.1101/2024.08.29.610246