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pHygGP16
(Plasmid #244903)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244903 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBluescript II SK (+)
  • Backbone size (bp) 3000
  • Modifications to backbone
    1) Removal of SapI site in pBluescript II SK (+), 2) Addition of a loxP site, MCS, FRT3 site, FRT site, rps-0 promoter, hygromycin resistance gene, unc-54 3' UTR, nls-GFP fluorescent protein and myo-2 3' UTR, 3) addition of attP site all via multi-insert Golden Gate Assembly.
  • Vector type
    Worm Expression ; Recombination Mediated Cassette Exchange; Recombination Mediated Homolog Exchange
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.01.583017 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHygGP16 was a gift from Michael Nonet (Addgene plasmid # 244903 ; http://n2t.net/addgene:244903 ; RRID:Addgene_244903)

  • For your References section:

    Creation and manipulation of bipartite expression transgenes in C. elegans using phiC31 recombinase. Nonet ML. bioRxiv 2024.03.01.583017 10.1101/2024.03.01.583017
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