pHygGP16
(Plasmid
#244903)
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Purpose(Empty Backbone) hygR selection nls-GFP marked rapid RMCE integration vector with an attP site for RMHE
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 244903 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepBluescript II SK (+)
- Backbone size (bp) 3000
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Modifications to backbone1) Removal of SapI site in pBluescript II SK (+), 2) Addition of a loxP site, MCS, FRT3 site, FRT site, rps-0 promoter, hygromycin resistance gene, unc-54 3' UTR, nls-GFP fluorescent protein and myo-2 3' UTR, 3) addition of attP site all via multi-insert Golden Gate Assembly.
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Vector typeWorm Expression ; Recombination Mediated Cassette Exchange; Recombination Mediated Homolog Exchange
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.03.01.583017 for the bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHygGP16 was a gift from Michael Nonet (Addgene plasmid # 244903 ; http://n2t.net/addgene:244903 ; RRID:Addgene_244903) -
For your References section:
Creation and manipulation of bipartite expression transgenes in C. elegans using phiC31 recombinase. Nonet ML. bioRxiv 2024.03.01.583017 10.1101/2024.03.01.583017