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Addgene

pAttBFNeo
(Plasmid #244923)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244923 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBluescript II derivative
  • Backbone size (bp) 2850
  • Modifications to backbone
    Addition of an FRT site, a Multiple Cloning Site with SapI sites for Golden Gate cloning, a lox2722 flanked rps-0 promoter NeoR unc-54 3' UTR cassette, and a phiC31 attB site.
  • Vector type
    Worm Expression ; phiC31 recombinase mediated insertion (RMI)
  • Promoter none
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.01.583017 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAttBFNeo was a gift from Michael Nonet (Addgene plasmid # 244923 ; http://n2t.net/addgene:244923 ; RRID:Addgene_244923)

  • For your References section:

    Creation and manipulation of bipartite expression transgenes in C. elegans using phiC31 recombinase. Nonet ML. bioRxiv 2024.03.01.583017 10.1101/2024.03.01.583017
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