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Addgene

pU6-BbsI-EGFR
(Plasmid #244932)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244932 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pU6-BbsI-chiRNA (Addgene #45946)
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA targeting EGFR C terminus
  • gRNA/shRNA sequence
    GAAACCGCAACACGGAGACG
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-BbsI-EGFR was a gift from Jared Toettcher (Addgene plasmid # 244932 ; http://n2t.net/addgene:244932 ; RRID:Addgene_244932)

  • For your References section:

    A live-cell biosensor of in vivo receptor tyrosine kinase activity reveals feedback regulation of a developmental gradient. Ho EK, Kim-Yip RP, Simpkins AG, Farahani PE, Oatman HR, Posfai E, Shvartsman SY, Toettcher JE. Cell Rep. 2025 Jul 22;44(7):115930. doi: 10.1016/j.celrep.2025.115930. Epub 2025 Jun 27. 10.1016/j.celrep.2025.115930 PubMed 40581929
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