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pG20_KEA1_TEV_HA_FR
(Plasmid #244961)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244961 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGreen2.0
  • Backbone size w/o insert (bp) 6069
  • Vector type
    Plant Expression
  • Selectable markers
    FAST-Red (mKate2)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kea1
  • Alt name
    At1g01790
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    6086
  • Entrez Gene
    KEA1 (a.k.a. AT1G01790, ATKEA1, K+ EFFLUX ANTIPORTER 1, K+ efflux antiporter 1, T1N6.21, T1N6_21)
  • Promoter UBQ10
  • Tag / Fusion Protein
    • TEV-HA (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pG20_KEA1_TEV_HA_FR was a gift from Hans-Henning Kunz (Addgene plasmid # 244961 ; http://n2t.net/addgene:244961 ; RRID:Addgene_244961)

  • For your References section:

    Catch & Release-rapid cost-effective protein purification from plants using a DIY GFP-Trap-protease approach. Schwartz S, Engstler C, Muhlbauer S, Windenbach E, Wunder T, Kunz HH, Brandt B. Plant J. 2025 Nov;124(3):e70544. doi: 10.1111/tpj.70544. 10.1111/tpj.70544 PubMed 41222448
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