ptwin strep HRV3C Flag Munc13-4 C2AB
(Plasmid
#245000)
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PurposeCalcium binding defective human Munc13-4 mutant
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245000 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneC1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUNC13D
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SpeciesH. sapiens (human)
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Entrez GeneUNC13D (a.k.a. FHL3, HLH3, HPLH3, Munc13-4)
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Tag
/ Fusion Protein
- twin-strep-HRV3C-Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. Addgene NGS finds four point mutations in UNC13D (NP_954712.1): D127N, D133N, D947N, D941N.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ptwin strep HRV3C Flag Munc13-4 C2AB was a gift from Thomas Martin (Addgene plasmid # 245000 ; http://n2t.net/addgene:245000 ; RRID:Addgene_245000)