Myc tagged SNAP25b Sequence2
(Plasmid
#245057)
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PurposeDistribution and localization of synthetic wild-type SNAP25 in cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 245057 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneC1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSNAP25
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SpeciesSynthetic
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This construct has an antibody binding domain flanked N terminally as well as internally tagged for detection of SNARE zippering
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Myc tagged SNAP25b Sequence2 was a gift from Thomas Martin (Addgene plasmid # 245057 ; http://n2t.net/addgene:245057 ; RRID:Addgene_245057)