twin-step HRV3C PLEKHM1
(Plasmid
#245058)
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PurposeExpression and purification of human wild-type PLEKHM1 in cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 245058 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneC1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePLEKHM1
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SpeciesH. sapiens (human)
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Entrez GenePLEKHM1 (a.k.a. AP162, B2, OPTA3, OPTB6)
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Tag
/ Fusion Protein
- twin-strep-HRV3C-Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
twin-step HRV3C PLEKHM1 was a gift from Thomas Martin (Addgene plasmid # 245058 ; http://n2t.net/addgene:245058 ; RRID:Addgene_245058)