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pAAV-hsynapsin-DIO-scramble shRNA - mCherry
(Plasmid #245064)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245064 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 5927
  • Vector type
    Mammalian Expression, AAV, RNAi, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAV-DIO-scramble-shRNA-mCherry
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
    2793
  • Promoter human synapsin
  • Tag / Fusion Protein
    • mCherry

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    As the basis for the shRNA expression from the mir30 locus, we used the pPRIME system (Addgene Plasmid 11664; gift from S. Elledge, Howard Hughes Medical Institute). We then transposed this cassette into an AAV transgene.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

These constructs appear in our manuscript:

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hsynapsin-DIO-scramble shRNA - mCherry was a gift from William Wisden (Addgene plasmid # 245064 ; http://n2t.net/addgene:245064 ; RRID:Addgene_245064)
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