pAAV-hsynapsin-DIO-scramble shRNA - mCherry
(Plasmid #245064)

• Purpose: AAV transgene designes to express scramble (control) shRNA sequence cell-type selectively. Scramble hairpin expressed from the mir30 cassette together with mCherry.
• Depositing Lab: William Wisden
• Publication: Anuncibay Soto et al Proc Natl Acad Sci U S A. 2025 Oct 14;122(41):e2422878122. doi: 10.1073/pnas.2422878122. Epub 2025 Oct 7.

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Backbone

• Vector backbone: pUC
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 5927
• Vector type: Mammalian Expression, AAV, RNAi, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AAV-DIO-scramble-shRNA-mCherry
• Species: M. musculus (mouse), Synthetic
• Insert Size (bp): 2793
• Promoter: human synapsin
• Tag / Fusion Protein:
  • mCherry

Cloning Information

• Cloning method: Other
• 5′ sequencing primer: cacgcgaggcgcgagatag

Resource Information

• A portion of this plasmid was derived from a plasmid made by: As the basis for the shRNA expression from the mir30 locus, we used the pPRIME system (Addgene Plasmid 11664; gift from S. Elledge, Howard Hughes Medical Institute). We then transposed this cassette into an AAV transgene.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

These constructs appear in our manuscript: