SIN-mGAD67-AcGFPnuc-WPRE
(Plasmid
#245086)
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PurposeLentiviral vector with nuclear fluorescent reporter AcGFP expressed from Mouse GAD67 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 245086 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneSIN-LV
- Backbone size w/o insert (bp) 7623
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAcGFP
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SpeciesSynthetic
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Insert Size (bp)5239
- Promoter Mouse GAD67 promoter
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Tag
/ Fusion Protein
- 3x SV40 NLS (C terminal on insert)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SIN-mGAD67-AcGFPnuc-WPRE was a gift from Nicole Déglon (Addgene plasmid # 245086 ; http://n2t.net/addgene:245086 ; RRID:Addgene_245086) -
For your References section:
Restricted transgene expression in the brain with cell-type specific neuronal promoters. Delzor A, Dufour N, Petit F, Guillermier M, Houitte D, Auregan G, Brouillet E, Hantraye P, Deglon N. Hum Gene Ther Methods. 2012 Aug;23(4):242-54. doi: 10.1089/hgtb.2012.073. Epub 2012 Aug 30. 10.1089/hgtb.2012.073 PubMed 22934828