Circuit C57
(Plasmid
#245097)
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PurposeModified Re-NF-FF controller with lower translational rate of integrase
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245097 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepSB3K3
- Backbone size w/o insert (bp) 2672
- Total vector size (bp) 8472
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Circuit C57 was a gift from Xiaojun Tian (Addgene plasmid # 245097 ; http://n2t.net/addgene:245097 ; RRID:Addgene_245097) -
For your References section:
Integrating recombinase-based feedback and feedforward control for optimal resource decoupling. Zhang R, Zhang R, Tian XJ. Nucleic Acids Res. 2025 Sep 5;53(17):gkaf913. doi: 10.1093/nar/gkaf913. 10.1093/nar/gkaf913 PubMed 40966512