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pLEW100v5-BSD-FLAG-La-Cas9
(Plasmid #245102)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245102 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLEW100v5-BSD
  • Vector type
    CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    3xFLAG-La-Cas9
  • Species
    Streptococcus pyogenes
  • Mutation
    codon optimized for T. brucei
  • Tag / Fusion Protein
    • 3xFLAG-La (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

contains constitutively expressed decoy guide with potential PAM included in guide sequence, digest with NotI for electroporation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEW100v5-BSD-FLAG-La-Cas9 was a gift from Monica Mugnier (Addgene plasmid # 245102 ; http://n2t.net/addgene:245102 ; RRID:Addgene_245102)

  • For your References section:

    DNA damage drives antigen diversification in Trypanosoma brucei. Smith JE, Wang KJ, Kennedy EM, Munday JC, Singer L, Hakim JMC, So J, Beaver AK, Magesh A, Gilligan-Steinberg SD, Zheng J, Zhang B, Moorthy DN, Brown ZE, Akin EH, Mwakibete L, McCulloch R, Mugnier MR. Nature (2026) 10.1038/s41586-026-10337-6
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