pSRG12 (eft-3p::24xGCN4::t2a::tagbfp::h2b::tbb-2 3’UTR)
(Plasmid #245120)

• Purpose: SunTag reporter for live imaging of translation (when combined with scFv::GFP expression) in C. elegans
• Depositing Lab: Suzan Ruijtenberg
• Publication: van der Salm et al Development. 2025 May 15;152(10):dev204435. doi: 10.1242/dev.204435. Epub 2025 May 19.

Backbone

• Vector backbone: pBSK
• Backbone size w/o insert (bp): 2862
• Total vector size (bp): 8398
• Vector type: Bacterial Expression, Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: eft-3p
• Alt name: Peft-3
• Species: C. elegans (nematode)
• Insert Size (bp): 609

Gene/Insert 2

• Gene/Insert name: 24xGCN4
• Alt name: Suntag epitopes
• Insert Size (bp): 1725

Gene/Insert 3

• Gene/Insert name: T2A
• Insert Size (bp): 51

Gene/Insert 4

• Gene/Insert name: TagBFP (GLO)
• Alt name: TagBFP
• Alt name: Blue fluorescent protein (germline optimized)
• Insert Size (bp): 781

Gene/Insert 5

• Gene/Insert name: H2B
• Species: C. elegans (nematode)
• Insert Size (bp): 473

Gene/Insert 6

• Gene/Insert name: tbb-2 3'UTR
• Alt name: TuBulin, Beta 2
• Insert Size (bp): 332

Gene/Insert 7

• Gene/Insert name: right recombination arm MosSCI cxTi10816
• Species: C. elegans (nematode)
• Insert Size (bp): 403

Gene/Insert 8

• Gene/Insert name: Left recombination arm MosSCI cxTi10816
• Species: C. elegans (nematode)
• Insert Size (bp): 607

Resource Information

• Supplemental Documents:
  • pSRG_12 sequenced.gb
• A portion of this plasmid was derived from a plasmid made by: 24xGCN4 is from Addgene plasmid #61058, a kind gift from Tanenbaum.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry