pSRG11 (eft-3p::scfv(glo)::gfp(smu-1 introns)::tbb-2 3’UTR)
(Plasmid #245121)

• Purpose: Optimized SunTag antibody for expression in C. elegans. Useful for visualization of translation of GCN4 encoding mRNAs or for visualization of GCN4-tagged proteins
• Depositing Lab: Suzan Ruijtenberg
• Publication: van der Salm et al Development. 2025 May 15;152(10):dev204435. doi: 10.1242/dev.204435. Epub 2025 May 19.

Backbone

• Vector backbone: pBSK
• Backbone size w/o insert (bp): 2855
• Total vector size (bp): 8923
• Vector type: Bacterial Expression, Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: eft-3p
• Alt name: Peft-3
• Species: C. elegans (nematode)
• Insert Size (bp): 609

Gene/Insert 2

• Gene/Insert name: scFv (GLO)
• Alt name: single chain variable fragment (germline optimized)
• Alt name: Suntag antibody
• Insert Size (bp): 940

Gene/Insert 3

• Gene/Insert name: GFP (GLO)
• Alt name: Green fluorescent protein (germline optimized)
• Insert Size (bp): 2899

Gene/Insert 4

• Gene/Insert name: tbb-2 3'UTR
• Alt name: TuBulin, Beta 2
• Species: C. elegans (nematode)
• Insert Size (bp): 332

Gene/Insert 5

• Gene/Insert name: left recombination arm MosSCI ttTi5605
• Species: C. elegans (nematode)
• Insert Size (bp): 646

Gene/Insert 6

• Gene/Insert name: right recombination arm MosSCI ttTi5605
• Insert Size (bp): 532

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The scFv is based on Addgene plasmid #60907, a kind gift from Tanenbaum. The GFP insert (with smu introns) is from pCFJ1243, a kind gift from Frokjaer-Jensen.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.