pET15b His SUMO mNeon R338A PPM1H
(Plasmid
#245124)
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PurposeExpresses His SUMO mNeon R338A PPM1H in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245124 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepET15b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHis SUMO mNeon (loop 1) R338A PPM1H
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SpeciesH. sapiens (human)
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Mutationchanged Arginine 338 to Alanine
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Entrez GenePPM1H (a.k.a. ARHCL1, NERPP-2C, URCC2)
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Tag
/ Fusion Protein
- His SUMO tagged with mNeon
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 promoter
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15b His SUMO mNeon R338A PPM1H was a gift from Suzanne Pfeffer (Addgene plasmid # 245124 ; http://n2t.net/addgene:245124 ; RRID:Addgene_245124) -
For your References section:
Allosteric regulation of the Golgi-localized PPM1H phosphatase by Rab GTPases modulates LRRK2 substrate dephosphorylation in Parkinson's disease. Adhikari A, Tripathi A, Chiang CY, Sherpa P, Pfeffer SR. J Biol Chem. 2025 Sep 3:110679. doi: 10.1016/j.jbc.2025.110679. 10.1016/j.jbc.2025.110679 PubMed 40912655