pPOE031
(Plasmid #245143)

• Purpose: Expression plasmid with the constitutive Pcwp2 promoter driving expression of the C. difficile secA2 gene and adding a C-term 3xHis tag
• Depositing Lab: Robert Fagan
• Publication: Fisher et al Microbiology (Reading). 2026 Feb;172(2). doi: 10.1099/mic.0.001665.

Backbone

• Vector backbone: pMTL960
• Backbone manufacturer: Nigel P Minton
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pcwp2-secA2-6xHis
• Species: Synthetic
• Promoter: Pcwp2
• Tag / Fusion Protein:
  • 6 x His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pPOE031.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry