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pJAK124
(Plasmid #245146)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245146 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMTLSC-7215
  • Backbone manufacturer
    Nigel P Minton
  • Vector type
    Bacterial knock-in

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pcwp2-mCherry
  • Species
    Synthetic
  • Promoter Pcwp2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pDSW1728 (10.1128/AEM.03446-14)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJAK124 was a gift from Robert Fagan (Addgene plasmid # 245146 ; http://n2t.net/addgene:245146 ; RRID:Addgene_245146)

  • For your References section:

    A set of genetic tools for use in Clostridioides difficile and related species. Fisher H, de Lussy Kubisa L, Jakhmola A, Walker E, Kirk JA, Oatley P, Chaudhuri RR, Douce GR, Ormsby MJ, Fagan RP. Microbiology (Reading). 2026 Feb;172(2). doi: 10.1099/mic.0.001665. 10.1099/mic.0.001665 PubMed 41712450
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