pJAK212
(Plasmid
#245160)
-
Purpose(Empty Backbone) pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 12 (AGTGTAACG)
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245160 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepMTLSC-7215
-
Backbone manufacturerNigel P Minton
-
Vector typeBacterial knock-in
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pJAK212 was a gift from Robert Fagan (Addgene plasmid # 245160 ; http://n2t.net/addgene:245160 ; RRID:Addgene_245160) -
For your References section:
A set of genetic tools for use in Clostridioides difficile and related species. Fisher H, de Lussy Kubisa L, Jakhmola A, Walker E, Kirk JA, Oatley P, Chaudhuri RR, Douce GR, Ormsby MJ, Fagan RP. Microbiology (Reading). 2026 Feb;172(2). doi: 10.1099/mic.0.001665. 10.1099/mic.0.001665 PubMed 41712450