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pET-SUMO-SHP2-C-SH2(E139D)-Avi
(Plasmid #245190)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245190 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SHP2 C-SH2
  • Species
    H. sapiens (human)
  • Mutation
    E139D
  • Tags / Fusion Proteins
    • His-SUMO (N terminal on insert)
    • Avi (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-SUMO-SHP2-C-SH2(E139D)-Avi was a gift from Neel Shah (Addgene plasmid # 245190 ; http://n2t.net/addgene:245190 ; RRID:Addgene_245190)

  • For your References section:

    The pathogenic T42A mutation in SHP2 rewires the interaction specificity of its N-terminal regulatory domain. van Vlimmeren AE, Voleti R, Chartier CA, Jiang Z, Karandur D, Humphries PA, Lo WL, Shah NH. Proc Natl Acad Sci U S A. 2024 Jul 23;121(30):e2407159121. doi: 10.1073/pnas.2407159121. Epub 2024 Jul 16. 10.1073/pnas.2407159121 PubMed 39012820
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