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pET-28b His6-TEV-SHP2-FL(R4A,R5A)
(Plasmid #245194)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245194 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SHP2 full-length
  • Species
    H. sapiens (human)
  • Mutation
    R4A,R5A
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28b His6-TEV-SHP2-FL(R4A,R5A) was a gift from Neel Shah (Addgene plasmid # 245194 ; http://n2t.net/addgene:245194 ; RRID:Addgene_245194)

  • For your References section:

    The pathogenic E139D mutation stabilizes a non-canonical active state of the multi-domain phosphatase SHP2. van Vlimmeren AE, Jiang Z, Karandur D, Applebaum Licht AT, Shah NH. bioRxiv [Preprint]. 2025 Jul 4:2025.07.02.662799. doi: 10.1101/2025.07.02.662799. 10.1101/2025.07.02.662799 PubMed 40631229
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