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pAdEasy-mRFP-gsk3 beta S9A
(Plasmid #24521)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 24521 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Stratagene, ATCC
  • Backbone size w/o insert (bp) 33414
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    S9A. Mutant version of GSK3 that lacks the inhibitory phosphorylation site.
  • Entrez Gene
  • Tag / Fusion Protein
    • mRFP (N terminal on insert)

Cloning Information

Resource Information

Depositor Comments

mRFP-GSK3beta S9A was cloned into pShuttle-CMV between KpnI and NotI then recombined by homologous recombination into pAdEasy-1.

AdEasy is a registered trademark of the Johns Hopkins University.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAdEasy-mRFP-gsk3 beta S9A was a gift from Torsten Wittmann (Addgene plasmid # 24521)
  • For your References section:

    GSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. Kumar P, Lyle KS, Gierke S, Matov A, Danuser G, Wittmann T. J Cell Biol. 2009 Mar 23. 184(6):895-908. 10.1083/jcb.200901042 PubMed 19289791