pWJ 1781 URA v-Src FL
(Plasmid
#245255)
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PurposeExpression of full-length viral Src in yeast
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 245255 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepWJ 1781
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFull-length viral Src
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SpeciesRous sarcoma virus
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Mutationwild-type
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWJ 1781 URA v-Src FL was a gift from Neel Shah (Addgene plasmid # 245255 ; http://n2t.net/addgene:245255 ; RRID:Addgene_245255) -
For your References section:
Deep mutational scanning of the multi-domain phosphatase SHP2 reveals mechanisms of regulation and pathogenicity. Jiang Z, van Vlimmeren AE, Karandur D, Semmelman A, Shah NH. Nat Commun. 2025 Jul 1;16(1):5464. doi: 10.1038/s41467-025-60641-4. 10.1038/s41467-025-60641-4 PubMed 40595497