pRDB_246
(Plasmid
#245333)
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PurposeCas12a CRISPRko positive control guide; targets CD47
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 245333 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepRDA_722
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgRNA CD47
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gRNA/shRNA sequenceUnspecified
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SpeciesSynthetic
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MutationNone
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Entrez GeneCD47 (a.k.a. IAP, MER6, OA3)
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRDB_246 was a gift from John Doench & David Root (Addgene plasmid # 245333 ; http://n2t.net/addgene:245333 ; RRID:Addgene_245333)