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pEF-cMyc-Rab3A(F59S)
(Plasmid #245357)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245357 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEF-cMyc-C1
  • Backbone size w/o insert (bp) 4658
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rab3a
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    669
  • Mutation
    F59S, lower effectors binding
  • Entrez Gene
    Rab3a (a.k.a. RAB3)
  • Promoter EF-1a
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF-cMyc-Rab3A(F59S) was a gift from Thomas Martin (Addgene plasmid # 245357 ; http://n2t.net/addgene:245357 ; RRID:Addgene_245357)
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