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pZNpF-∆48∆11
(Plasmid #245369)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245369 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTwist-EF1a
  • Backbone manufacturer
    Twist Biosciences
  • Vector type
    Mammalian Expression, Luciferase, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zfp423-∆48∆11
  • Species
    M. musculus (mouse)
  • Mutation
    11-bp deletion, H96Wfs*4, plus synthetic 48-bp upstream deletion to match ∆59 cds length prior to stop
  • Entrez Gene
    Zfp423 (a.k.a. Ebfaz, Roaz, Zfp104, Znf423, ataxia1, mKIAA0760, nur12)
  • Promoter EF1a
  • Tag / Fusion Protein
    • NanoLuciferase (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

clone 6.2

5' cloning site: EcoRI. 3' cloning site: BamHI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZNpF-∆48∆11 was a gift from Bruce Hamilton (Addgene plasmid # 245369 ; http://n2t.net/addgene:245369 ; RRID:Addgene_245369)

  • For your References section:

    Nonequivalence of Zfp423 premature termination codons in mice. Concepcion D, Liang C, Kim D, Hamilton BA. bioRxiv [Preprint]. 2025 Jun 2:2025.05.30.656936. doi: 10.1101/2025.05.30.656936. 10.1101/2025.05.30.656936 PubMed 40501617
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