pZNpF-deleted
(Plasmid
#245372)
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Purpose(Empty Backbone) Negative control, luciferase cassette deleted
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 245372 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepTwist-EF1a
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Backbone manufacturerTwist Biosciences
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Vector typeMammalian Expression, Synthetic Biology
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
clone 1.1
5' cloning site: EcoRI. 3' cloning site: BamHI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZNpF-deleted was a gift from Bruce Hamilton (Addgene plasmid # 245372 ; http://n2t.net/addgene:245372 ; RRID:Addgene_245372) -
For your References section:
Nonequivalence of Zfp423 premature termination codons in mice. Concepcion D, Liang C, Kim D, Hamilton BA. bioRxiv [Preprint]. 2025 Jun 2:2025.05.30.656936. doi: 10.1101/2025.05.30.656936. 10.1101/2025.05.30.656936 PubMed 40501617