pATUBR
(Plasmid
#245488)
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PurposeBinary Plasmid for plant transformation expressing RUBY from the Arabidopsis thaliana polyubiquitin 10 gene promoter and the terminator from the pea RBCS gene. Kanamycin plant selection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245488 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepAGM4273
- Backbone size w/o insert (bp) 12773
- Total vector size (bp) 12236
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepAtUBI-RUBY-tRBCS
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Insert Size (bp)6117
- Promoter Polyubiquitin 10 gene from Arabidopsis thaliana
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer gacgtcgttgtggttggtgc (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pATUBR was a gift from Beth Rowan (Addgene plasmid # 245488 ; http://n2t.net/addgene:245488 ; RRID:Addgene_245488)
Map uploaded by the depositor.
