pLSUBR
(Plasmid #245489)

• Purpose: Binary Plasmid for plant transformation expressing RUBY from the Lactuca sativa polyubiquitin 4 gene promoter and the terminator from the same gene. Kanamycin plant selection
• Depositing Lab: Beth Rowan
• Publication: Monitoring the stability of transgene expression in lettuce using the RUBY reporter. (unpublished)

Backbone

• Vector backbone: pAGM4273
• Backbone manufacturer: Sylvestre Marillonet
• Backbone size w/o insert (bp): 12773
• Total vector size (bp): 12559
• Vector type: Plant Expression
• Selectable markers: kanamycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pLsUBI-RUBY-tLSUBI
• Insert Size (bp): 6440
• Promoter: Polyubiquitin 4 gene (LOC111919935) from lettuce

Cloning Information

• Cloning method: Golden Gate
• 5′ sequencing primer: CGGTTTTTCAGTGACCTCTCTAAAG

Resource Information

• Supplemental Documents:
  • pl2b-lsubr.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry